Doctors and patients at a Nottingham practice must find a new clinic after a landlord refused use of the building for legal reasons.
Around 2,000 people are patients at St Ann's Phoenix Practice, but they must now find a new surgery after the owner of the building ordered doctors and staff to leave.
Although the closure had been expected, practice manager Sue Whiteman said: "We weren't surprised, but it's never nice to hear about the final chop."
Despite efforts to find a new location, there were no other suitable alternative premises in the area.
No jobs will be lost because of the closure and Ms Whiteman said patients would be reassigned to different surgeries in the area.
Michelle Rhodes, director of delivery and performance at NHS Nottingham City, said: "Unfortunately, despite ongoing negotiations with the landlord, we have been unable to agree a satisfactory way forward for patients at this current address.
"There are around 2,000 patients registered at the Phoenix Practice. We are very sorry for the inconvenience caused to them by this situation, but we are confident that this process will be as smooth as possible and people will still have good access to high-quality local health services."